子宫肌瘤引起的不孕症求子十部曲 子宮肌瘤引起的不孕症求子十部曲 https://www.facebook.com/yes7260678/posts/10151490196336877 根據統計,在台灣地區,35歲以上的婦女,每4個人就有一個人罹患子宮肌瘤,其實子宮肌瘤在女人的子宮非常常見,子宮肌瘤就是從子宮肌肉層長出來的一種腫瘤,它大部分是良性的,因為它裡面有肌纖維,所以稱子宮纖維腫。有子宮肌瘤並不代表不孕症,要看肌瘤長的位置,如果長在子宮表面的漿膜層,小小的肌瘤,並不會影響受孕,如果子宮肌瘤長在肌肉層裡面,會影響子宮內腔的變形,這就會不孕,如果是長在子宮的內黏膜下,。凸出子宮腔的話,這就會妨礙受孕了。 婦女有子宮肌瘤,但她們已經有小孩子又有子宮肌瘤是不太一樣的,如果不想生育的話,只要把整個子宮摘除就好了,可是如果有子宮肌瘤又還沒生孩子,或者有生孩子又想再生第二個孩子,那不能把子宮摘除,必須把肌瘤挖掉。子宮肌瘤不代表不孕症,有很多婦女子宮長了肌瘤照樣生兩個、三個,所以子宮肌瘤會不會不孕是看肌瘤的位置,位置比大小重要,如果只長在子宮頸下方或輸卵管、子宮腔內部分,就會阻塞輸卵管,或讓精子沒辦法游入子宮,當然就會不孕,但是如果肌瘤長在子宮的表面,那就不礙事了,也不會不孕症,有的子宮肌瘤是長在肌肉層而影起子宮的血流、子宮內膜的機能,因此會影響胚胎的著床,或讓病人經血過多,這也會容易不孕症或容易流產。不孕症病人有肌瘤的話,有的人要開刀切除,有的人可以放著不管,子宮肌瘤引起的不孕症,要看是什麼位置引起的不孕症,一般來說子宮肌瘤如果引起的不孕症,經過肌瘤切除或摘除之後,受孕的機會增加,手術後兩年之內有百分之25到百分之50的受孕率,當然受孕後的流產率比一般婦女高,一旦妳有子宮肌瘤,又懷孕後需接受剖腹產,因為妳的子宮有開刀傷口,以下是我建議的子宮肌瘤引起不孕症婦女的求子十部曲: (1)如果有月經來有疼痛或經血量過多或摸都可以摸得子宮肌瘤(太大了),妳必須找婦產科醫師做個超音波,看是否有子宮肌瘤、位置在哪裡,在子宮表面?肌肉層?或黏膜層?它的大小是幾公分? (2)若是子宮肌瘤在子宮表面,也就是所謂漿膜層,那沒關係,如果不想開刀也可以,如果是屬於巨大的肌瘤,則建議先開刀,以避免懷孕之後因為子宮增大,而造成肌瘤壓迫到腸胃道,或橫隔膜,造成心肺部的壓迫。 (3)如果子宮肌瘤在肌肉層或比較裡面的黏膜層,則必須建議開刀來增加受孕率,以減少流產率,如果子宮肌肉層或黏膜層的肌瘤不摘除,容易會有月經血過多,流血過多而貧血,所以為了求子、為了健康妳必須進行手術,切除肌瘤。 (4)如果是子宮肌肉層的肌瘤,可以透過先進腹腔鏡手術或剖腹手術,把整個子宮肌瘤摘除再縫合,這個手術流血量會比較大,也會引起手術之後的腸沾黏,因此可以考慮在手術之前,在子宮肌瘤旁邊注射血管收縮劑,這樣可以減少開刀的流血量,開完子宮肌瘤之後,可以在子宮的表面覆蓋一層減少腸沾黏的保護膜(Interseed),這樣可以減少手術之後的沾黏,而引起輸卵管阻塞的不孕症,否則子宮肌瘤解決了,但又增加一個輸卵管阻塞、骨盆腔沾黏的不孕症。 (5)如果是子宮黏膜層的肌瘤,若超過5公分必須在手術之前先打3-6個月的腦下垂體協同劑的迪波(長效型),打完3-6個月之後,肌瘤會縮小,再安排全身麻醉的TCR(子宮鏡手術)肌瘤摘除術,手術時間比較久,而為了避免子宮穿破,必須在做子宮鏡的同時,架設腹腔鏡來監測子宮鏡手術,以避免子宮穿孔。 (6)黏膜層肌瘤:於摘除之後必須使用3個月高劑量的女性賀爾蒙,使子宮內膜復原,這樣才會增加胚胎著床的機率,子宮肌瘤手術前後的準備盡量周延,不可以急於懷孕,而使的整個肌瘤得摘除,與復原工程沒有完成會得不償失,一方面不容易懷孕,二方面容易流產。 (7)當妳完成子宮肌瘤的診治之後,再來要確定妳的卵巢功能與輸卵管的通暢性,才來決定求子的途徑,若子宮肌瘤合併子宮內膜異位症,而引起的輸卵管沾黏,則必須進行試管嬰兒,試管嬰兒療程為月經第21天開始施打腦下垂體協同劑,在月經第二天抽血及格之後,於月經第三天開始施打8-12天的排卵針,打排卵針等到濾泡成熟後,再施打破卵針HCG,打破卵針之後34-36小時後取卵,取卵之後3-5天把胚胎植入到子宮,植入子宮之後如驗孕有了,恭喜妳有了,再在過7天可以由陰道超音波看到胚囊,又在過7天,可以由陰道超音波看到胎兒心跳,看到胎兒心跳,看到胎兒心跳妳就可以比較叫放心了。 (8)子宮肌瘤摘除之後,輸卵管也通,而妳的年紀超過35歲,在37歲半以前,妳可以考慮做幾次人工受精,人工受精的作法是,妳於月經第三天開始施打排卵針劑,於月經第8天開始回醫院測量濾泡的成熟度,等到卵泡成熟,施打破卵針之後,24、48小時做兩次人工受精(有人在36小時做一次),做完人工受精之後,妳必須接受14天的黃體素支持療法,14天驗小便若呈現陽性反應,抽血數值超150,恭喜妳有了可能一胞胎,超過350可能雙胞胎,又在過另7天可以從陰道超音波看到胚囊,在過7天可從陰道超音波聽到小孩子的心跳。 (9)若子宮肌瘤整治工程完畢,妳的年紀為37歲半到40歲以上,或都還沒生過小孩子,則建議進入試管嬰兒療程,試管嬰兒療程事先服用一個月的避孕藥,於月經來的第21天開始施打腦下垂體協同劑,等到月經來第二天抽血賀爾蒙(LH、E2、P4)如及格的話,於月經第三天開始施打排卵針劑,等到卵泡成熟,打破卵針之後的34-36小時取卵,取完卵之後3-5天把胚胎植入子宮,14天之後驗孕,恭喜妳有了,其他療程比照(8)。 (10)若是妳的子宮肌瘤過大,在手術的時候沒辦法完全子宮肌瘤切除,或妳的子宮肌瘤是屬於多發型的子宮肌瘤,也就是一個子宮裡面可能有十幾二十顆大大小小的肌瘤,而醫師在開刀當中,研判必須把子宮肌瘤連同子宮一併摘除,而妳又完全沒生過小孩子,子宮摘除是為了避免妳經血過多而貧血,導致右心臟衰竭而不得不做的子宮摘除術,妳也簽下手術同意書,醫師也摘除了妳的子宮,妳還是一樣可以生孩子,不過必須進行向別的女人借子宮的代理孕母,目前台灣代理孕母還沒有合法化,立法院還沒有通過,因此妳要代理孕母必須要遠赴重洋,到美國,在美國 加州地區有不少代理孕母公司執行這項業務,我們有病人到美國去執行這項業務,花費在200-400萬台幣不等,因此如果妳是比較罕見的子宮摘除的不孕症病人,妳可能要有心理準備與這筆預算,子宮是別人的但是卵子是妳的,精子是妳丈夫的,所以生下來的孩子也是妳與妳丈夫愛的結晶,妳不必太過於沮喪。 透過以上十部曲,妳有子宮肌瘤不管大小,妳仍然可以擁有一子,祝妳心想事成、早日求子成功 本文與彰化有線第26台彰視新聞台,每週四晚上九點到十點『做人真簡單』現場同步撥出,如果您閱讀本文章有任何疑問,歡迎於每週四晚上九點到十點現場call in,電話:8235858 附加檔案:子宮肌瘤容易引起不孕症 作者 彰化市博元不孕症試管嬰兒中心 院長蔡鋒博
- Mar 27 Fri 2015 14:19
子宫肌瘤引起的不孕症求子十部曲
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- 2015: 胚胎快篩:一條龍試管嬰兒 整合醫療團隊訂做優生寶寶
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future. 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR <iframe src='https://www.shakr.com/embed/sBCI6Jwgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> <iframe src='https://www.shakr.com/embed/8WI8Z9kgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be <iframe width="560" height="315" src="https://www.youtube.com/embed/oeUzkFgSyww" frameborder="0" allowfullscreen></iframe> 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future. 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR <iframe src='https://www.shakr.com/embed/sBCI6Jwgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> <iframe src='https://www.shakr.com/embed/8WI8Z9kgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be <iframe width="560" height="315" src="https://www.youtube.com/embed/oeUzkFgSyww" frameborder="0" allowfullscreen></iframe> 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future. 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR <iframe src='https://www.shakr.com/embed/sBCI6Jwgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> <iframe src='https://www.shakr.com/embed/8WI8Z9kgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be <iframe width="560" height="315" src="https://www.youtube.com/embed/oeUzkFgSyww" frameborder="0" allowfullscreen></iframe> 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future. 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR <iframe src='https://www.shakr.com/embed/sBCI6Jwgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> <iframe src='https://www.shakr.com/embed/8WI8Z9kgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be <iframe width="560" height="315" src="https://www.youtube.com/embed/oeUzkFgSyww" frameborder="0" allowfullscreen></iframe> 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future. 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR <iframe src='https://www.shakr.com/embed/sBCI6Jwgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> <iframe src='https://www.shakr.com/embed/8WI8Z9kgv-A' width='640' height='360' frameborder='0' allowfullscreen webkitallowfullscreen mozallowfullscreen></iframe> https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be <iframe width="560" height="315" src="https://www.youtube.com/embed/oeUzkFgSyww" frameborder="0" allowfullscreen></iframe> 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 台視新聞報導博元婦產科一條龍試管嬰兒 TTV qPCR https://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be 新聞報導博元婦產科一條龍試管嬰兒 TTV qPCRhttps://www.youtube.com/watch?v=oeUzkFgSyww&feature=youtu.be
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future.
- 2015: 14歲前 打子宮頸疫苗 可少一劑 患者年輕化 及早防癌省4千
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。
- 2015: pgs 胚胎快篩:著床前基因診斷世界新趨勢 selection embryo
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future.
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future.
- 2015: 婦產科醫學年會先驅性研究:胚胎快篩 「一條龍式試管嬰兒」助好孕 在2015年3月15日的婦產科醫學年會中,有許多新技術及眾多學術論文發表,其中彰化博元婦產科蔡鋒博醫師發表的「一條龍式試管嬰兒」研究更是受人矚目。 國內目前的試管嬰兒技術已經相當成熟,懷孕率與歐美並駕齊驅。 目前有待突破的關鍵點在於如何選擇健康的胚胎植回子宮, 以及在胚胎著床階段究竟受何因素的影響,如胚胎著床之窗, 這些議題依舊是醫界努力研究的領域。 蔡醫師說明,現在的試管嬰兒技術, 傳統是應用光學顯微鏡觀察,依據胚胎外觀形態來選擇胚胎,目測的結果當然也有可能選錯胚胎,比如:外觀優質,但染色體異常。正常健康的胚胎有23對染色體,分別從精子及卵細胞各取一條染色體,共46條染色體。 如何選擇染色體正常的整倍胚胎、選到最有潛力著床的胚胎, 是試管嬰兒治療成功最關鍵的步驟。 染色體不整倍將導致胚胎無法著床或流產, 這也是在試管嬰兒治療中,最需要被克服的問題之一。 在「一條龍式試管嬰兒」研究中,是將試管嬰兒囊胚期胚胎切片以qPCR(即時定量聚合酶連鎖反應儀)進行PGS胚胎著床前染色體診斷, 因為胚胎快篩:胚胎切片檢測染色體較快速,所以可以不必為了等待判讀結果而冷凍胚胎,可以直接新鮮胚胎植入子宮。 在發表的先驅性的臨床研究中,三個月內收集了15個案例,總共分析了163個囊胚,不整倍的染色體胚胎占39.2%,扣除不整倍的染色體胚胎後,整倍體胚胎著床率為:53.8%。 初步結果有相當好的懷孕率及胚胎的著床率,15個案例中有11例懷孕,因病人數較少,不能遽下結論, 仍須收集更多案例,才能提供更有力的臨床證據。 蔡醫師表示,在試管嬰兒的治療中,選擇染色體正常的胚胎可望提高懷孕率、 降低流產率,而以qPCR進行胚胎快篩染色體之胚胎快篩「一條龍式試管嬰兒」技術的應用, 將對於有基因遺傳疾病、反覆流產的病人有相當大的幫忙。 Application of q-PCR to preimplantation genetic screening: A pilot study 試管嬰兒囊胚期胚胎切片以qPCR進行PGS胚胎著床前染色體診斷,不必冷凍,直接新鮮胚胎植入子宮:先驅性研究 蔡鋒博*1, 陳昭雯1, 林招彰1, 張舜評2,3, 馬國欽 2, ,張月嬌1,陳曉青1,徐慧鈴1,潘孟麗1,張琇媛1,施俐君1陳明 2,4,5 Feng-Po Tsai1, Chao-Wen Chen1, Chao-Chang Lin1, Shun-Ping Chang2,3, Gwo-Chin Ma2, Yueh-Chiao Chang1,Hsiao-Ching Chen1,Hui-Ling Hsu1,Meng-LI Pan1,Hsiu-Yuan Chang1,Li-Chun Shih1, Ming Chen2,4,5 Poyuan Women Clinic IVF Centre, Changhua Taiwan1 博元婦產科,不孕症試管嬰兒中心[1] 彰化基督教醫院 基因醫學部[2] 中興大學 生命科學系[3] 台灣大學醫學院婦產部[4] 東海大學 生命科學系[5] Introduction Only a small fraction of embryos that can successfully implant and progress to live-birth in spite of recent advances in morphology-based embryological methodologies, selection of competent embryos is therefore a crucial step in IVF treatment. Aneuploidy is regarded as a primary etiology of implantation failure and it is vitally important to develop an effective strategy to solve this deficiency. Various measures including FISH, array CGH (SNP or oligo chromosomal microarray), and q-PCR have been reported. Among them, q-PCR seems a very promising new modality in PGS by a small number of IVF centers abroad. This pilot study aimed at analyzing the impact on implantation rate and pregnancy rate in IVF cycles using fresh embryos transfer which embryos were selected via blastocyst biopsy with rapid qPCR-based partial aneuploidy screening in our setting. It has been shown that biopsy at blastocyst stage is less detrimental in implantation potential than biopsy at Day-3 cleavage stage embryos and thus in this study, we tested a simple strategy by using q-PCR on Day5/6 blastocyst stage embryos and assessed its outcome. Patient(s) Material and method 15 infertile couples with at least one previous IVF failure came to our clinic were enrolled. PGS was conducted by q-PCR with selectively amplified markers situated at chromosome 13, 18, 21, and X. The blastocyst biopsy was performed on day 5/6 and the sample was analyzed on the same day. The results were immediately available to the embryologist and the clinician. The euploid embryo was subsequently transferred in the fresh cycle. Result(s) A total of 163 blastocysts were biopsied and 4.9% of embryos (n=8) generated no result due to amplification failure. The aneuploidy rate for blastocysts was 39.2% (n=64). 26 blastocysts were transferred and implantation rate was 53.8% (n=14). Clinical pregnancy rate was 73.3% (n=11). Conclusion Our pilot study showed a promising high pregnancy rate and implantation rate. Despite it is yet comprehensive chromosome screening aiming at all 24 chromosomes, the strategy combines the traditional methodology by FISH and the biopsy of blastocyst followed by fresh embryo transfer, which gains an advantage of biopsy of Day-3 cleavage stage embryos. Expanding the chromosomes being investigated (from a limited set of chromosomes to all 24 chromosomes) is underway and a randomized study by comparing q-PCR and array CGH is needed in the future.
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- 2014: 如何達到試管嬰兒成功率極高!
- 2014: 年紀與不孕症的關係,為什麼40歲以後要做試管嬰兒?age
- 2014: 如何從胚胎外觀去判斷胚胎染色體正常與否?pgs
- 2014: 為什麼做試管嬰兒要取到15~20顆卵子成功率最高?!EGG
- 2014: 在博元婦產科借卵試管嬰兒一次成功生雙胞胎!DONATION egg
- 2014: 新鮮胚胎和冷凍胚胎合併植入子宮,成功懷孕博元做一次試管嬰兒成功 PGS cryo un
- 2014: 如何達到試管嬰兒成功率極高!pgs
- 2014: 冷凍胚胎,在博元一次試管嬰兒成功 cryo
- 2014: 博元婦產科 播豬哥亮秀仿效;國外新療法 做試管嬰兒看笑片
- 2014: 48歲試管嬰兒3胞胎〈中天新聞〉報導博元試管嬰兒中心
- 2014: 卵巢扭轉-年代
- 2014: 在博元婦產科借卵一次成功 終結4 個先天疾病小孩的噩夢!
- 2014: 36歲在博元只做一次試管嬰兒,就成功生下一對雙胞胎
- 2011: 多次試管嬰兒失敗新救星 搔刮子宮內膜做試管嬰兒成功懷孕案例 <a href="http://www.flickr.com/photos/41541241@N08/5485463389/" title="Flickr 上 7260678 的 搔刮子宮內膜"><img src="http://farm6.static.flickr.com/5093/5485463389_26dba0bb58.jpg" width="260" height="194" alt="搔刮子宮內膜" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5486058880/" title="Flickr 上 7260678 的 搔刮子宮內膜2"><img src="http://farm6.static.flickr.com/5292/5486058880_7f3b378683.jpg" width="398" height="243" alt="搔刮子宮內膜2" /></a> <object width="960" height="750"><param name="movie" value="http://www.youtube.com/v/FTTPfUl2AmY?fs=1&hl=zh_TW"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/FTTPfUl2AmY?fs=1&hl=zh_TW" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="960" height="750"></embed></object> <object width="960" height="750"><param name="movie" value="http://www.youtube.com/v/mFrf2eyxTeI?fs=1&hl=zh_TW"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/mFrf2eyxTeI?fs=1&hl=zh_TW" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="960" height="750"></embed></object> 進行試管嬰兒療程,進入誘導排卵療程前先予以輕度搔刮子宮內膜,因為子宮內膜修補的過程中會分泌大量的生長因子與細胞激素,而這些生長因子與細胞激素是胚胎著床所須的。接著進行試管嬰兒療程,植入四個高品質的胚胎,結果順利懷了雙胞胎! 有沒有子宮刮燒做試管嬰兒成功懷孕案例 請問蔡醫師你最近回答病人網友說刮燒子宮會增加試管嬰兒成功率,請問你有成功的案例嗎? 小豆子你好:博元婦產科在做試管嬰兒前一個月會幫病人做子宮鏡,如果有問題都會幫病人做息肉切除,切除就是子宮刮燒,因此成功 的個案很多很多,結一次來講,王小姐因為在別的醫院做過2次試管嬰兒失敗,在我們醫院要做之前幫他做子宮鏡,發現他有很多息肉,經過息肉切除之後,一次試管嬰兒成功生下雙胞龍鳳胎,請看以下的報導。 以色列Nava博士研究發現,在胚胎適合著床期間,子宮內膜的生化表現,和子宮內膜受傷誘發的發炎反應極為類似,受傷的子宮內膜會釋放出腫瘤壞死因子(TNF-α),刺激產生許多細胞間質(Cytokines),引來吞噬細胞及大量神經樹突細胞的聚集。而這些免疫細胞促使子宮內膜細胞產生微小分子,進而和早期胚胎(囊胚)互動,引導它向子宮內膜接觸、結合進而著床。Nava博士的研究證實子宮內膜受刺激後,做試管嬰兒有兩倍以上的成功率。 板主 於 2011/2/18 上午 08:45 回覆 「子宮內膜刺激術」,乃是以人為的方式讓子宮內膜產生小傷口,引發體內生長因子聚集在子宮,除了幫助傷口復原,在未來的兩三個月內,生長因子也會協助胚胎著床在子宮內膜上,可以提高試管嬰兒治療成功率, http://www.youtube.com/watch?v=Sdv0PJvkum8&feature=player_embedded <object width="640" height="390"><param name="movie" value="http://www.youtube.com/v/Sdv0PJvkum8&hl=en_US&feature=player_embedded&version=3"></param><param name="allowFullScreen" value="true"></param><param name="allowScriptAccess" value="always"></param><embed src="http://www.youtube.com/v/Sdv0PJvkum8&hl=en_US&feature=player_embedded&version=3" type="application/x-shockwave-flash" allowfullscreen="true" allowScriptAccess="always" width="640" height="390"></embed></object> 多次試管嬰兒失敗新救星以色列醫師Ravit,發現進入試管前一個月幫病患做子宮鏡及子宮內膜刺激術,可使胚胎重覆著床失敗的病人成功受孕。 他發現19位多次試管失敗患者,接受這種治療後再做試管嬰兒,懷孕率高達42%,另外的19位患者,因為沒做子宮鏡合併子宮內膜刺激術,沒有一位成功懷孕。 Ravit認為子宮內膜刺激術,使患者子宮自行產生對胚胎著床有幫助的細胞間素及生長 因子,透過這些因子使胚胎和子宮內膜之間,取得良好的「溝通」並進一步著床! 博元婦產科不孕症試管嬰兒中心:蔡鋒博,陳昭雯醫師
- 2011: 黃體軟膏優於黃體打針crinone <a href="http://www.flickr.com/photos/41541241@N08/5546291344/" title="Flickr 上 7260678 的 黃體素不足成功生子 2"><img src="http://farm6.static.flickr.com/5136/5546291344_2567d552b7.jpg" width="400" height="300" alt="黃體素不足成功生子 2" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5546165649/" title="Flickr 上 7260678 的 黃體油針"><img src="http://farm6.static.flickr.com/5300/5546165649_c2ed6c2157.jpg" width="480" height="360" alt="黃體油針" /></a> 最近有一份研究針對哈佛醫學院馬克醫師,他用試管嬰兒隨機分成兩組,一組是打油針很痛,一組是用黃體軟膏快孕隆,在206位用黃體軟膏裡面有93個懷孕成功,201位打黃體油針的病人,有85位懷孕成功,這樣算起來成功率快孕隆這一組是45%,黃體油針這一組是43%,這是一樣的,那既然懷孕率一樣,為什麼不選比較不痛的快孕隆。
- 2011: 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做試管嬰兒(中天新聞) 日本有一位鈴木先生,因為台灣籍的妻子要做試管嬰兒,就怕妻子在日本,受孕的胚胎會受到輻射污染影響,因此鈴木先生陪著太太,回到彰化博元婦產科,進行受孕手術,雖然鈴木先生必須先拋下日本的教職工作,但鈴木先生說,太太和孩子的健康,才是最重要的。 <a href="http://www.flickr.com/photos/41541241@N08/5560654192/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)4"><img src="http://farm6.static.flickr.com/5096/5560654192_dc0f76ffb3_b.jpg" width="720" height="480" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)4" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5560082425/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)22"><img src="http://farm6.static.flickr.com/5024/5560082425_77ebb45f8c_b.jpg" width="720" height="480" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)22" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5560082709/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)21"><img src="http://farm6.static.flickr.com/5142/5560082709_ea710c33af_b.jpg" width="720" height="480" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)21" /></a> http://www.youtube.com/watch?v=BcWHswK9ftg <object width="400" height="298" ><param name="allowfullscreen" value="true" /><param name="movie" value="http://www.facebook.com/v/10150131266388363" /><embed src="http://www.facebook.com/v/10150131266388363" type="application/x-shockwave-flash" allowfullscreen="true" width="400" height="298"></embed></object> <iframe title="YouTube video player" width="960" height="750" src="http://www.youtube.com/embed/BcWHswK9ftg" frameborder="0" allowfullscreen></iframe> 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞) 日本有一位鈴木先生,因為台灣籍的妻子要做試管嬰兒,就怕妻子在日本,受孕的胚胎會受到輻射污染影響,因此鈴木先生陪著太太,回到彰化博元婦產科,進行受孕手術,雖然鈴木先生必須先拋下日本的教職工作,但鈴木先生說,太太和孩子的健康,才是最重要的。 <a href="http://www.flickr.com/photos/41541241@N08/5558367174/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)1"><img src="http://farm6.static.flickr.com/5140/5558367174_f9ece2b982.jpg" width="500" height="333" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)1" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5558366854/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)3"><img src="http://farm6.static.flickr.com/5136/5558366854_9739d4091e.jpg" width="500" height="333" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)3" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5558365570/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)10"><img src="http://farm6.static.flickr.com/5179/5558365570_d636cb8700.jpg" width="500" height="333" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)10" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5558367016/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)2"><img src="http://farm6.static.flickr.com/5016/5558367016_2498953535.jpg" width="500" height="333" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)2" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5558365570/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)10"><img src="http://farm6.static.flickr.com/5179/5558365570_d636cb8700.jpg" width="500" height="333" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)10" /></a> <a href="http://www.flickr.com/photos/41541241@N08/5557779701/" title="Flickr 上 7260678 的 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)18"><img src="http://farm6.static.flickr.com/5109/5557779701_021db60aa2.jpg" width="500" height="333" alt="怕輻射,日教授陪妻特地搭機來博元婦產科受孕做人工授精(中天新聞)18" /></a> 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做試管嬰兒(中天新聞) 日本有一位鈴木先生,因為台灣籍的妻子要做試管嬰兒,就怕妻子在日本,受孕的胚胎會受到輻射污染影響,因此鈴木先生陪著太太,回到彰化博元婦產科,進行受孕手術,雖然鈴木先生必須先拋下日本的教職工作,但鈴木先生說,太太和孩子的健康,才是最重要的。
- 2011: 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做試管嬰兒(中天新聞)
- 2011: 怕輻射,日教授陪妻特地搭機來博元婦產科受孕做試管嬰兒(中天新聞)
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